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Toxicity of fatty acid 18:5n3 from gymnodinium cf. mikimotoi : II. intracellular pH and K + uptake in isolated trout hepatocytes
Author(s) -
Fossat Barthélémy,
PorthéNibelle Jacqueline,
Sola François,
Masoni Armand,
Gentien Patrick,
Bodennec Guy
Publication year - 1999
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199907/08)19:4<275::aid-jat578>3.0.co;2-b
Subject(s) - intracellular , extracellular , trout , toxicity , biochemistry , intracellular ph , polyunsaturated fatty acid , biology , atpase , fatty acid , chemistry , enzyme , fishery , fish <actinopterygii> , organic chemistry
Effects of octadecapentaenoic acid 18:5n3 and other related polyunsaturated fatty acids present in Gymnodinium cf. mikimotoi were tested in isolated trout hepatocytes. These exotoxins decreased intracellular pH followed by a slow recovery to initial value and alkalinization of acidic compartments, suggesting an inhibition of vacuolar H + ‐ATPases. Moreover, addition of 18:5n3 to the extracellular medium induced a decrease of K + uptake into hepatocytes as a result of Na,K‐ATPase inhibition. However, high concentrations (10 −5 –10 −3 M) are necessary to induce these effects. Copyright © 1999 John Wiley & Sons, Ltd.