Premium
Comparison of cytotoxicity of various surfactants tested on normal human fibroblast cultures using the neutral red test, MTT assay and LDH release
Author(s) -
Arechabala B.,
Coiffard C.,
Rivalland P.,
Coiffard L. J. M.,
RoeckHoltzhauer Y. De
Publication year - 1999
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199905/06)19:3<163::aid-jat561>3.0.co;2-h
Subject(s) - neutral red , pulmonary surfactant , cytotoxicity , chemistry , lactate dehydrogenase , chromatography , cationic polymerization , sodium , alcohol dehydrogenase , mtt assay , chloride , biochemistry , ethanol , enzyme , in vitro , organic chemistry
We used the neutral red test, MTT assay and lactate dehydrogenase (LDH) release to compare the potential cytotoxicity of six surfactants belonging to different classes—three non‐ionic surfactants (Triton ×100, octylphenoxypolyethoxy alcohol, from Orion; Tween 60, polyoxyethylene (20) sorbitan monostearate, from ICI Speciality Chemicals; Tween 80, polyoxyethylene (20) sorbitan monolaurate, from Labosi), two anionic surfactants (Texapon K1298, sodium lauryl sulphate, from Henkel; Texapon N40, sodium laurylether sulphate, from Henkel) and one cationic surfactant (benzethonium chloride, from Siber Hegner)—on human fibroblast cultures. According to the LC 50 (μg ml −1 ), the tested surfactants can be classified in the following order of increasing cytotoxicity: Tween 80 < Texapon N40 < Tween 60 < Texapon K1298 < Triton ×100 < benzethonium chloride. Copyright © 1999 John Wiley & Sons, Ltd.