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Investigations on the in vitro and in vivo genotoxic potential of 5‐vinyl‐2‐norbornene
Author(s) -
Vergnes Jane S.,
Ballantyne Bryan
Publication year - 1998
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199803/04)18:2<129::aid-jat484>3.0.co;2-c
Subject(s) - genotoxicity , sister chromatid exchange , chinese hamster , andrology , in vivo , chemistry , chromosome aberration , bone marrow , gene mutation , microbiology and biotechnology , in vitro , biology , toxicity , medicine , immunology , genetics , biochemistry , mutation , chromosome , gene
5‐Vinyl‐2‐norbornene (VNB: CAS no. 3048‐64‐4), an industrial chemical that produces hyaline droplet nephropathy in the male rat with associated α 2u ‐globulin increases, was investigated in vitro and in vivo for its genotoxic potential. A Salmonella typhimurium reverse mutation assay (strains TA98, 100, 1535, 1537, 1538) was negative both without (dose range 0.003–0.3 mg per plate) and with (0.003–0.3 mg per plate) metabolic activation. A forward gene mutation test in Chinese Hamster Ovary (CHO) cells (HGPRT locus) did not show any significant concentration‐related increases in mutation frequencies in the absence (0.01–0.1 mg ml −1 ) or presence (0.005–0.1 mg ml −1 ) of metabolic activation. In a sister chromatid exchange (SCE) test, VNB did not produce statistically significant or dose‐related increases in the incidence of SCEs in the absence (0.02–0.06 mg ml −1 ) or presence (0.005–0.03 mg ml −1 ) of metabolic activation. A bone marrow chromosome aberration test was conducted in groups of 10 male and 10 female Sprague–Dawley rats exposed for 6 hr/day for 5 consecutive days to mean (± SD) VNB vapor concentrations of 0 (air control), 48.1 ± 1.29, 146 ± 9.2, or 336 ± 8.5 ppm. Marrow was collected 6 and 24 hr after the final exposure. No statistically significant or dose‐related increases in chromosomal aberrations occurred in the VNB‐exposed animals. 5‐Vinyl‐2‐norbornene did not show any potential for genotoxic activity with this in vitro–in vivo battery of tests. © 1998 John Wiley & Sons, Ltd.