Premium
Rhein affects arylamine N ‐acetyltransferase activity in Helicobacter pylori from peptic ulcer patients
Author(s) -
Chung Jing G.,
Tsou Mei F.,
Wang Hwang H.,
Lo Hsueh H.,
Hsieh Sue E.,
Yen Yee S.,
Wu Lii T.,
Chang Shih H.,
Ho Chin C.,
Hung Chi F.
Publication year - 1998
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199803/04)18:2<117::aid-jat486>3.0.co;2-d
Subject(s) - helicobacter pylori , n acetyltransferase , bacteria , acetylation , chemistry , acetyltransferase , peptic ulcer , arylamine n acetyltransferase , radioimmunoassay , cytosol , growth inhibition , microbiology and biotechnology , biochemistry , enzyme , medicine , biology , in vitro , genetics , gene
Arylamine N ‐acetyltransferase (NAT) activities with 2‐aminofluorene and p ‐aminobenzoic acid were determined in the bacterium Helicobacter pylori collected from peptic ulcer patients. Cytosols or suspensions of H. pylori with or without specific concentrations of rhein co‐treatment showed different percentages of 2‐aminofluorene and p ‐aminobenzoic acid acetylation. The data indicate that there was decreased NAT activity associated with increased levels of rhein in H. pylori cytosols. Inhibition of growth studies from H. pylori demonstrated that rhein elicited dose‐dependent bacteriostatic activity in H. pylori cultures: i.e. the greater the concentration of rhein, the greater the inhibition of growth to H. pylori . For the cytosol and intact bacteria examination, the apparent values of K m and V max were decreased after co‐treatment with 40 μM rhein. This report is the first demonstration of rhein inhibition of arylamine N ‐acetyltransferase activity and rhein inhibition of growth in the bacterium H. pylori . © 1998 John Wiley & Sons, Ltd.