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Rat Embryo Culture Using Rabbit Serum as a Medium for Developmental Toxicity Studies
Author(s) -
Nakajima Mikio,
Sasaki Masanori,
Kobayashi Youshiro,
Ohno Yasuo,
Usami Makoto
Publication year - 1997
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199705)17:3<185::aid-jat428>3.0.co;2-#
Subject(s) - embryo , rabbit (cipher) , biology , embryo culture , andrology , toxicity , gestation , lagomorpha , endocrinology , medicine , embryogenesis , pregnancy , microbiology and biotechnology , genetics , statistics , mathematics
The usefulness of rabbit serum as a culture medium for postimplantation rat embryos was examined. Rat embryos at day 9.5 of gestation were cultured in a mixture of rat and rabbit sera at various ratios (v/v) for 48 h. In 100% rat serum, a usual medium, rat embryos grew well. On the contrary, rat embryos died with little growth in 100% rabbit serum. In 75% rabbit and 25% rat sera, rat embryos grew but were morphologically abnormal. In 50% rabbit and 50% rat serum, however, rat embryos grew well showing no morphological abnormalities, as in 100% rat serum. It was concluded from these results that rabbit serum could be used at a proportion up to 50% as a medium for postimplantation rat embryo culture in a mixed form with rat serum. The rat embryo culture using rabbit serum as a medium would be useful in developmental toxicity studies, especially those involving species differences and toxicokinetics. © 1997 John Wiley & Sons, Ltd.