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Effect of Topically Applied Sulphur Mustard on Antioxidant Enzymes in Blood Cells and Body Tissues of Rats
Author(s) -
Husain K.,
Dube S. N.,
Sugendran K.,
Singh R.,
Gupta S. Das,
Somani S. M.
Publication year - 1996
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199605)16:3<245::aid-jat339>3.0.co;2-3
Subject(s) - superoxide dismutase , catalase , spleen , antioxidant , glutathione peroxidase , glutathione , chemistry , sulfur mustard , kidney , biochemistry , pharmacology , toxicity , enzyme , endocrinology , medicine , immunology , biology , organic chemistry
The effect of sulphur mustard (0.5 LD 50 , percutaneous) on antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH‐Px)) in blood cells (erythrocytes (RBC), leucocytes (WBC) and platelets) and body tissues (liver, kidney, spleen and brain) of rats has been investigated 24 h post exposure. The SOD activity was significantly decreased in WBC, platelets, spleen and brain as compared to control. The CAT activity was significantly inhibited in RBC, WBC and spleen as compared to control. The GSH‐Px activity was significantly depressed in WBC, spleen and liver as compared to control. It is concluded that sulphur mustard at a sublethal dose inhibited antioxidant enzyme activities in WBC and spleen. Thus, antioxidant enzymes in lymphatic tissues may be used as suitable models for assessing mustard toxicity. The study suggests the formation of reactive oxygen species in sulphur mustard intoxication.