Premium
Development and Validation of a High‐performance Liquid Chromatography Method for the Determination of Cocaine, its Metabolites and Lidocaine
Author(s) -
Barat Stephen A.,
Kardos Stacey A.,
AbdelRahman Mohamed S.
Publication year - 1996
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199605)16:3<215::aid-jat335>3.0.co;2-i
Subject(s) - benzoylecgonine , lidocaine , chromatography , chemistry , high performance liquid chromatography , acetonitrile , aqueous solution , metabolite , anesthesia , organic chemistry , medicine , biochemistry
Street cocaine varies in purity and is often adulterated with various compounds. Some of these additives, such as lidocaine, may increase the toxicity of cocaine. A new precise, accurate and sensitive reversed‐phase high‐performance liquid chromatography method for the determination of cocaine, its metabolites and lidocaine in plasma samples has been developed and validated. This assay employed a phosphate‐buffered aqueous mobile phase (pH 6.0) with an organic component consisting of acetonitrile and methanol and a C‐18 column as the stationary phase. Minimum detection limits were 1 ng ml −1 for cocaine, 2.5 ng ml −1 for ethylcocaine and 5 ng ml −1 for benzoylecgonine, norcocaine, norethylcocaine and lidocaine. Linearity was demonstrated over a broad range of concentrations in plasma, with good sensitivity for cocaine and cocaine derivatives.