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Cytogenetic Effect of Griseofulvin in Mouse Spermatocytes
Author(s) -
Fahmy Maha A.,
Hassan Nagwa H. A.
Publication year - 1996
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199603)16:2<177::aid-jat330>3.0.co;2-t
Subject(s) - griseofulvin , meiosis , ploidy , metaphase , biology , andrology , genetics , chromosome , toxicology , medicine , dermatology , gene
The genotoxic effects of griseofulvin (GF) in mouse primary spermatocytes at diakinesis metaphase I of meiosis were investigated. Griseofulvin was administered orally as a single dose of 500, 1000, 1500 and 2000 mg kg−1 body wt. and a multiple treatment with a daily dose of 1000 mg kg−1 body wt. for three and five successive doses. Both single and multiple treatment induced a statistically significant increase in the percentage of chromosomal aberrations which have a dose and time‐dependent relationship. The frequency of chromosomal aberrations peaked 6 and 12 h post treatment; with the highest dose of the drug it reached 27.8% ± 0.87 and 27.66% ± 0.48 6 and 12 h respectively, compared with 5.6% ± 0.39 and 5.2% ± 0.48 for the control. The types of aberrations recorded were structural, including X–Y and autosomal univalent, gaps, breaks, fragments, chain IV and numerical in the form of diploid, triploid, tetraploid and aneuploid. The results of this study suggest that griseofulvin has a genotoxic effect in mouse spermatocytes.