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Chronic Oral Toxicity of a Synthetic Antioxidant, 2,2′‐Methylenebis(4‐ethyl‐6‐ tert ‐butylphenol), in Rats
Author(s) -
Takagi Atsuya,
Takada Koichi,
Sai Kimie,
Momma Junko,
Aida Yoshitaka,
Suzuki Sachiko,
Naitoh Katsushi,
Tobe Masuo,
Hasegawa Ryuichi,
Kurokawa Yuji
Publication year - 1996
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/(sici)1099-1263(199601)16:1<15::aid-jat288>3.0.co;2-n
Subject(s) - vacuolization , toxicity , medicine , endocrinology , adverse effect , blood urea nitrogen , body weight , no observed adverse effect level , oral administration , antioxidant , kidney , urea , physiology , chemistry , biochemistry
Groups of 30 Wistar rats of each sex were treated with 2,2′‐methylenebis(4‐ethyl‐6‐tert‐butylphenol) (MBEBP) in the diet at levels of 0, 0.03, 0.1 and 0.3% for up to 18 months. In both sexes, survival rates of treated groups were similar to those of the controls. Body weight gain was depressed (0.3% group in males, 0.1 and 0.3% groups in females). Slight anaemia (0.3% groups in both sexes) and increase of blood urea nitrogen (0.3% groups in both sexes) were observed. Histopathologically, vacuolization of the parathyroid gland cells (0.3% group in males and all treated groups in females) and degenerative changes of the kidney (0.1 and 0.3% groups in males) were observed. No neoplastic responses following MBEBP administration were noted. From these results, the no‐observed‐adverse‐effect level (NOAEL) for MBEBP toxicity was estimated as 12 mg kg−1 body wt. day−1 in male rats. In female rats, the lowest‐observed‐adverse‐effect level (LOAEL) was estimated as 15 mg kg−1 body wt. day−1.