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Molecular detection of a B cell clone in a case of PTCL in the absence of T cell clonality
Author(s) -
Smith J. L.,
Hodges E.,
Quin C. T.,
Wright D. H.
Publication year - 1997
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/(sici)1099-1069(199705)15:2<63::aid-hon599>3.0.co;2-w
Subject(s) - clone (java method) , biology , t cell receptor , gene rearrangement , polyclonal antibodies , polymerase chain reaction , microbiology and biotechnology , southern blot , antibody , population , t cell , lymphoma , virology , gene , immunology , medicine , immune system , genetics , environmental health
In this paper we document a case of peripheral T cell lymphoma (PTCL) that exhibited variable T cell histology at presentation and follow‐up. Southern blot analysis for T cell receptor (TCR) and immunoglobulin (Ig) receptor gene rearrangements failed to reveal clonal T or B cell populations. TCRγ (TCRG) and β (TCRB) chain gene polymerase chain reaction (PCR) amplification of DNA isolated from biopsies was also consistent with polyclonal T cell populations, however Ig PCR revealed clonal Ig rearrangements in follow‐up biopsies but not in the presentation biopsy. There was no histological evidence for a neoplastic B cell population in these biopsies although occasional EB virus positive blasts were present. The significance of a cryptic B cell clone is unknown but suggests a relationship with the proliferating polyclonal T cells in this case of PTCL. These data reflect the complexity of PTCL with implications for treatment and patient management. © 1997 John Wiley & Sons, Ltd.