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Different effects of cyclic AMP and butyrate on eosinophilic differentiation, apoptosis and bcl‐2 expression of a human eosinophilic leukemia cell line, EoL‐1
Author(s) -
Guixiang Tai,
EunYoung Jung,
Yuji Horiguchi,
Masahiko Kawai,
Toshio Heike,
Kenji Katamura,
Mitsufumi Mayumi
Publication year - 1996
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/(sici)1099-1069(199612)14:4<181::aid-hon589>3.0.co;2-y
Subject(s) - eosinophilic , butyrate , apoptosis , cancer research , leukemia , cell culture , medicine , chemistry , pathology , biology , immunology , biochemistry , genetics , fermentation
A human eosinophilic leukemia cell line, EoL‐1, stopped proliferating at the G 1 phase, differentiated into eosinophilic granule‐containing cells, and died by apoptosis when stimulated with dibutyryl cyclic AMP (dbcAMP). To clarify the effects of dbcAMP, the effects of butyrate and cAMP‐increasing reagents, prostaglandin E 2 (PGE 2 ) and forskolin, on EoL‐1 cellular differentiation and apoptosis were examined and compared. PGE 2 and forskolin but not butyrate induced differentiation to eosinophilic granule‐containing cells, suggesting that cAMP played a primary role in eosinophilic differentiation of EoL‐1 cells. PGE 2 , forskolin and butyrate, when used alone, did not induce apoptosis of EoL‐1 cells significantly at the concentrations used, but sequential stimulation of EoL‐1 cells with the cAMP‐increasing reagents and butyrate showed that butyrate induced further maturation and apoptosis of cAMP‐induced eosinophilic granule‐containing cells. These results showed that cAMP and butyrate have different effects on eosinophilic differentiation and apoptosis of EoL‐1 cells. The cAMP‐increasing reagents and butyrate also showed different effects on expression of members of the bcl‐2 family; PGE 2 decreased bcl‐2 and bax levels, whereas butyrate increased the bcl‐2 level. PGE 2 or PGE 2 +butyrate, but not butyrate alone, induced bcl‐x S expression. EoL‐1 cells constitutively expressed Fas and anti‐Fas antibody induced EoL‐1 cell death, but the Fas/Fas ligand system was not involved in dbcAMP‐induced EoL‐1 cell apoptosis. The EoL‐1 cell line is thus a useful model in which to examine differentiation and apoptosis of eosinophilic leukemia cells. © 1996 John Wiley & Sons, Ltd.