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Developmental changes in capacitative Ca 2+ entry in mouse mammary epithelial cells
Author(s) -
Ichikawa Junm,
Furuya Kishio,
Miyata Seiji,
Nakashima Toshihiro,
Kiyohara Toshikazu
Publication year - 2000
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(200006)18:2<147::aid-cbf864>3.0.co;2-w
Subject(s) - thapsigargin , intracellular , extracellular , endocrinology , fura 2 , medicine , pregnancy , chemistry , calcium , microbiology and biotechnology , biology , biochemistry , cytosol , enzyme , genetics
Developmental changes in capacitative Ca 2+ entry and Ca 2+ release from intracellular stores were measured using fura‐2 fluorescence method during the pregnancy period (day 3—;18) in mouse mammary epithelial cells. Ca 2+ release was identified with the transient intracellular Ca 2+ ([Ca 2+ ] i ) increase induced by thapsigargin addition in a Ca 2+ ‐free solution. Capacitative Ca 2+ entry was measured by the transient [Ca 2+ ] i increase induced by re‐addition of extracellular Ca 2+ after depletion of Ca 2+ stores by thapsigargin. The capacitative Ca 2+ entry was greatest at the early stage of pregnancy (i.e. day 3 of pregnancy) and decreased as pregnancy progressed, while Ca 2+ release remained unchanged throughout the developmental stages. These findings indicate that in contrast to Ca 2+ release, a close correlation exists between capacitative Ca 2+ entry and pregnancy‐induced development in mammary epithelial cells. Copyright © 2000 John Wiley & Sons, Ltd.