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Protective effects of vitamin E on carbon tetrachloride‐induced liver damage in rats
Author(s) -
Naziroğlu Mustafa,
Çay Mehmet,
Üstündağ Bilal,
Aksakal Mesut,
Yekeler Hayrettin
Publication year - 1999
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199912)17:4<253::aid-cbf837>3.0.co;2-r
Subject(s) - carbon tetrachloride , medicine , vitamin e , endocrinology , alkaline phosphatase , vitamin , lactate dehydrogenase , ccl4 , cirrhosis , necrosis , chemistry , antioxidant , biochemistry , enzyme , organic chemistry
In this study we investigated whether the increase of hepatic vitamin E content by intraperitoneal administration, influences chronic liver damage induced by carbon tetrachloride (CCl 4 ) in rats. Thirty adult male Wistar rats were divided into three groups. The first group was used as a control and the rats in the second group were administered CCl 4 in olive oil subcutaneously. Rats in the third group were administered intraperitoneally vitamin E (dl‐α‐tocopherol acetate, 100 mg kg −1 ). This administration was performed three times per week for five weeks. Liver samples were used for the determination of vitamin E levels, glutathione peroxidase (GSHPx) activities and histological examination. Serum levels of alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase, aspartate aminotransferase, gamma‐glutamyltranspeptidase, total and conjugated bilirubin were significantly ( p <0·05, p <0·01, p <0·001) higher in animals treated with CCl 4 than in the controls and had returned to normal values by the administration of vitamin E + CCl 4 . Liver vitamin E levels were significantly ( p <0·05) lower in the CCl 4 group than in the control group. However, the liver vitamin E content was significantly ( p <0·01, p <0·001) increased in the vitamin E + CCl 4 injected group. On the other hand, liver GSHPx activity was not statistically different among the groups. On histological examination, vitamin E administered animals showed incomplete, but significant, prevention of liver necrosis and cirrhosis induced by CCl 4 . these data indicate that intraperitoneally administered vitamin E has protective effects against CCl 4 ‐induced chronic liver damage and cirrhosis as evidenced by biochemical data and conventional histological examination. Copyright © 1999 John Wiley & Sons, Ltd.