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An improved histochemical method for measuring nitric oxide synthase activity
Author(s) -
AlaghbandZadeh Jamshid,
Mehdizadeh Siroos,
O'Farrell Anne,
Weisz Judith,
Bitensky Lucille,
Chayen Joseph
Publication year - 1999
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199909)17:3<217::aid-cbf826>3.0.co;2-m
Subject(s) - nitric oxide synthase , reagent , chemistry , nitric oxide , biochemistry , citrate synthase , atp synthase , nuclear chemistry , enzyme , organic chemistry
The previous quantitative histochemical method for measuring nitric oxide synthase (NOS) activity in tissue sections involved the loss of about 15 per cent of the NOS, presumably from the section into the reaction medium. Two changes are now described. The first is concerned with the preparation in the laboratory of the active reagent, lead ammonium citrate/acetate (LACA). The second change involves an improvement of the procedure for measuring NOS activity. The new method appears to retain all the measurable NOS activity inside the section. Copyright © 1999 John Wiley & Sons, Ltd.