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NADPH‐oxidase activity and lipid peroxidation in neutrophils from rats fed fat‐rich diets
Author(s) -
Lopes Lucia R.,
Laurindo Francisco R. M.,
ManciniFilho Jorge,
Curi Rui,
Sannomiya Paulina
Publication year - 1999
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199903)17:1<57::aid-cbf811>3.0.co;2-r
Subject(s) - lipid peroxidation , coconut oil , superoxide dismutase , catalase , chemistry , thiobarbituric acid , soybean oil , food science , arachidonic acid , glutathione peroxidase , biochemistry , glycogen , endocrinology , medicine , biology , antioxidant , enzyme
In order to investigate the effect of fat‐rich diets on neutrophil functions, 21 day‐aged rats were fed for 6 weeks with a control diet consisting of a regular laboratory rodent chow (4 per cent final fat content), a control diet supplied with soybean oil (15 per cent final fat content), or a control diet supplied with coconut oil (15 per cent final fat content). Glycogen‐elicited peritoneal neutrophils from rats fed soybean and coconut oil‐enriched diets presented a reduction in spontaneous and PMA‐stimulated H 2 O 2 generation relative to neutrophils from rats fed the control diet. The activity of superoxide dismutase, glutathione peroxidase and catalase did not change in animals fed fat‐rich diets. In addition, the capacity to generate O ·− 2 spontaneously or in response to PMA, did not change in neutrophils from animals fed fat‐rich diets. Values attained matched those observed in animals fed the control diet, regardless of the method used to measure O ·− 2 the superoxide dismutase‐inhibitable reduction of cytochrome c or the lucigenin‐dependent chemiluminescence. However, the initial rate of O ·− 2 generation both in resting neutrophils and in PMA‐stimulated cells was significantly reduced when animals were fed with coconut or soybean oil‐enriched diets due, at least in part, to a reduction in the activity of glucose‐6‐phosphate dehydrogenase. The concentration of thiobarbituric acid reactive substances, an index of lipid peroxidation, was increased in animals fed both fat‐rich diets. This was accompanied by an increase in arachidonic acid content in these cells. Results presented suggest that lipid peroxidation in neutrophils from animals fed fat‐rich diets may be associated with a consumption of H 2 O 2 yielding more reactive oxygen‐derived species such as the hydroxyl radical. Copyright © 1999 John Wiley & Sons, Ltd.