Premium
Oxidative activity in mitochondria isolated from rat liver at different stages of development
Author(s) -
Iossa Susanna,
Lionetti Lillà,
Mollica Maria Pina,
Barletta Antonio,
Liverini Giovanna
Publication year - 1998
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(1998120)16:4<261::aid-cbf796>3.0.co;2-1
Subject(s) - mitochondrion , oxidative phosphorylation , medicine , endocrinology , respiration , body weight , biology , respiratory system , nad+ kinase , oxidative stress , biochemistry , enzyme , anatomy
The purpose of this study was to evaluate the oxidative capacities in hepatic mitochondria isolated from prepubertal, young adult and adult rats (40, 90 and 180 days of age, respectively). In these rats, mitochondrial respiratory rates using FAD‐ and NAD‐linked substrates as well as mitochondrial protein mass were measured. The results show that only the oxidative capacity of FAD‐linked pathways significantly declined in mitochondria from 180‐day‐old rats compared with those from younger animals. When we consider FAD‐linked respiration expressed per g liver, no significant difference was found among rats of different ages because of an increased mitochondrial protein mass found in 180‐day‐old rats. However, when FAD‐linked and lipid‐dependent respiratory rates were expressed per 100 g body weight, significant decreases occurred in 180‐day‐old rats. Therefore, the decrease in liver weight expressed per 100 g body weight rather than an impaired hepatic cellular activity may be the cause of body energy deficit in 180‐day‐old rats. Copyright © 1998 John Wiley & Sons, Ltd.