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Oxidized low‐density lipoprotein decreases the induced nitric oxide synthesis in rat mesangial cells
Author(s) -
Wu ZhaoLong,
Liang MingYu,
Qiu LianQun
Publication year - 1998
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199809)16:3<153::aid-cbf778>3.0.co;2-t
Subject(s) - nitric oxide , mesangial cell , medicine , endocrinology , chemistry , reverse transcription polymerase chain reaction , nitric oxide synthase , lipoprotein , messenger rna , microbiology and biotechnology , biochemistry , cholesterol , biology , gene , kidney
Recently, the close relation between oxidized low density lipoprotein (Ox‐LDL) and the progression of glomerular injury has been demonstrated. The nitric oxide (NO) pathway in glomerular mesangial cells may be a potential target for the adverse effects of Ox‐LDL in the development of glomerular injury. In this study, we treated cultured rat mesangial cells (RMC) with Fe 2+ ‐oxidized LDL and then stimulated the cells with lipopolysacharride (LPS, 10 μg ml −1 ). The LPS‐induced NO production, assessed by NO 2 − concentrations in cultured supernatants, decreased from 7·83 nmol per 10 6 cells in control to 4·00 nmol per 10 6 cells and 1·67 nmol per 10 6 cells in RMC preincubated with Ox‐LDL at 20 μg ml −1 and 40 μg ml −1 , respectively ( P <0·01). Native LDL had no significant effects on LPS‐induced NO production. Using the reverse transcription‐polymerase chain reaction (RT‐PCR) technique, we could not detect significant alteration of inducible NO synthase (iNOS) mRNA levels in RMC preincubated with Ox‐LDL. Our results suggest that Ox‐LDL decreases induced NO production in RMC, which may contribute to the adverse effects of Ox‐LDL in progressive glomerular injury. The mechanisms of this decrease may not involve changes of iNOS genic transcription. © 1998 John Wiley & Sons, Ltd.