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Effects of tumor necrosis factor α (TNFα) on the phospholipid metabolism of Tetrahymena pyriformis
Author(s) -
Kovács P.,
Köhidai L.,
Csaba G.
Publication year - 1998
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199806)16:2<87::aid-cbf770>3.0.co;2-i
Subject(s) - phosphatidic acid , phospholipid , sphingomyelin , inositol , biochemistry , ceramide , tetrahymena pyriformis , metabolism , arachidonic acid , biology , palmitic acid , lipid metabolism , chemistry , fatty acid , tetrahymena , enzyme , cholesterol , apoptosis , receptor , membrane
The effect of (0·05 ng ml −1 and 0·1 ng ml −1 ) TNFα on the phospholipid metabolism of Tetrahymena pyriformis was studied. The amount of phosphatidyl choline (PC), phosphatidyl inositol (PI), phosphatidic acid (PA), phosphatidyl ethanolamine (PE), diacylglycerol (DAG), arachidonic acid (AA) and ceramide was higher, but the phosphatidyl inositol 4 phosphate (PIP) and phosphatidyl inositol bis‐phosphate (PIP 2 ) as well, as sphingomyelin (SM) content was lower in TNFα‐treated cells than in the controls. In the culture medium (secreted forms) this situation was reversed. There were differences in the results gained by incorporation of [ 3 H]‐palmitic acid or 32 P into the phospholipids. To control the functional effects of TNFα in Tetrahymena , the rate of cell division, the condensation of chromatin, the viability of cells and morphometrical values have been studied. The cytokine reduced cell growth, altered morphometric indices and increased chromatin condensation, however cell viability was not influenced. The results demonstrate the effects of TNFα at a low level of evolution, what is realized by changes in the phospolipid metabolism participating in signalling pathways. © 1998 John Wiley & Sons, Ltd.