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Oxygen free radical scavenger properties of dehydroepiandrosterone
Author(s) -
Tamagno Elena,
Aragno Manuela,
Boccuzzi Giuseppe,
Gallo Marco,
Parola Silvia,
Fubini Bice,
Poli Giuseppe,
Danni Oliviero
Publication year - 1998
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199803)16:1<57::aid-cbf771>3.0.co;2-s
Subject(s) - chemistry , microsome , dehydroepiandrosterone , lipid peroxidation , hypoxanthine , xanthine oxidase , superoxide , radical , deoxyribose , antioxidant , scavenger , microsoma , biochemistry , free radical scavenger , oxidase test , hydroxyl radical , superoxide radical , medicine , enzyme , androgen , nucleic acid , hormone
The microsomes from dehydroepiandrosterone (DHEA)‐supplemented animals are good hydroxyl radical scavengers, as demonstrated through electron spin resonance and deoxyribose degradation. The ability of DHEA‐supplemented microsomes to react with superoxide radical was also demonstrated through the inhibition of nitro‐blue tetrazolium reduction determined by superoxide radicals produced in a hypoxanthine–xanthine oxidase system. DHEA‐enriched microsomes, obtained from acutely DHEA‐treated rats, become resistant to iron‐dependent lipid peroxidation triggered by H 2 O 2 /FeSO 4 and ascorbate/FeSO 4 . The direct addition of DHEA to microsomes from untreated rats failed to prevent iron‐dependent lipid peroxidation, even if the microsomes were preincubated with DHEA for up to 15 min, indicating that in vivo transformation is required before antioxidant action can be exerted. © 1998 John Wiley & Sons, Ltd.