Premium
Functional expression of basolateral Cl − /HCO 3 − exchange from rat jejunum in Xenopus laevis oocytes
Author(s) -
Tosco Marisa,
Orsenigo Maria novella,
Gastaldi Giulia,
Faelli Alide
Publication year - 1998
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199803)16:1<35::aid-cbf759>3.0.co;2-e
Subject(s) - dids , xenopus , antiporter , chemistry , messenger rna , microbiology and biotechnology , biochemistry , biophysics , biology , gene , membrane
Poly(A) + RNA isolated from rat jejunum was injected into Xenopus laevis oocytes and expression of Cl − /HCO 3 − antiport was investigated by means of 36 Cl − uptake. Two days after injection of 50 ng of poly(A) + RNA, Cl − uptake was significantly increased with respect to water‐injected oocytes. The expressed transport was inhibited by 0·2 m M DIDS, whereas endogenous Cl − uptake was unaffected by this disulphonic stilbene. After sucrose density gradient fractionation, the highest expression of DIDS‐sensitive Cl − uptake was detected with mRNA size fraction of about 2–4 kb in length. The expressed Cl − uptake can occur against a Cl − concentration gradient and is unaffected by the known Cl − channel blocker anthracene‐9‐carboxylic acid. Cl − transport mechanism has properties similar to jejunal basolateral Cl − /HCO 3 − exchange with regard to Na + dependence. © 1998 John Wiley & Sons, Ltd.