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Temperature dependency of calcium responses in mammary tumour cells
Author(s) -
Oosawa Yoshio,
Imada Chiharu,
Furuya Kishio
Publication year - 1997
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(19970601)15:2<113::aid-cbf730>3.0.co;2-x
Subject(s) - dependency (uml) , calcium , cancer research , microbiology and biotechnology , chemistry , biology , computational biology , computer science , medicine , artificial intelligence
Changes of intracellular calcium concentration ([Ca 2+ ] i ) induced by the extracellular application of ATP and bradykinin in mouse mammary tumour cells (MMT060562) were investigated by image analysis of fluo‐3 fluorescence at 24°C and 35°C. ATP (0·1–100 μ M ) and bradykinin (0·1 n M –1 μ M ) induced the increase of [Ca 2+ ] i at both temperatures and Ca 2+ ‐depletion did not affect these [Ca 2+ ] i responses. Both [Ca 2+ ] i responses became more sensitive at 35°C than at 24°C. A clear latency of [Ca 2+ ] i increased after the application of the agonists was observed, and it changed with the concentration of the agonist. As concentrations of ATP or bradykinin became lower, the latency and rise time became longer. At higher concentrations, the latency and rise time approached a constant value. The latency shortened remarkably at 35°C. These results suggested the involvement of a regenerative or threshold process in the [Ca 2+ ] i responses in mammary tumour cells. © 1997 John Wiley & Sons, Ltd.