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Involvement of Polyamines in the Action of Transforming Growth Factor β and Interleukin‐1 on Cultured Chick Embryo Fibroblasts
Author(s) -
EVANGELISTI R.,
VALENO V.,
BODO M.,
BOSI G.,
STABELLINI G.,
CARINCI P.
Publication year - 1997
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199703)15:1<47::aid-cbf719>3.0.co;2-f
Subject(s) - polyamine , transforming growth factor , cell growth , dna synthesis , embryo , thymidine , transforming growth factor beta , stimulation , biology , microbiology and biotechnology , growth factor , endocrinology , medicine , dna , biochemistry , receptor
In this study we have examined the relationship between growth factor‐induced proliferation and ODC/polyamine levels. TGFβ promotes cell growth and enhances [ 3 H]‐thymidine incorporation in chick embryo fibroblasts maintained in a serum‐depleted medium. The action on DNA synthesis declines in the second day of treatment. IL‐1 does not affect proliferation or [ 3 H]‐thymidine incorporation either when it is added alone or in combination with TGFβ. The response of the cells to TGFβ is associated with a significant stimulation of ODC activity and Put, Spd levels together with an enhancement of the Spd/polyamines ratio. IL‐1, which does not act on cell proliferation, fails to activate ODC and to increase polyamine levels, thus indicating that the ODC/polyamine system is most likely to be an important link in the chain of events that leads to growth factor‐induced proliferation. © 1997 John Wiley & Sons, Ltd.