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The Effect In vitro of High‐Density Lipoprotein from Healthy and Infected Humans on the Oxidative Metabolism of Polymorphonuclear Leukocytes
Author(s) -
FURLANETO CRISTIANE,
CAMPA ANA
Publication year - 1997
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/(sici)1099-0844(199703)15:1<39::aid-cbf718>3.0.co;2-d
Subject(s) - superoxide , lucigenin , respiratory burst , oxidative metabolism , chemiluminescence , oxidative phosphorylation , myeloperoxidase , chemistry , in vitro , stimulation , luminol , metabolism , granulocyte , phorbol , low density lipoprotein , lipoprotein , superoxide dismutase , zymosan , biochemistry , oxidative stress , endocrinology , cholesterol , inflammation , biology , immunology , enzyme , protein kinase c , organic chemistry
We studied the effects in vitro of high‐density lipoprotein from healthy (N‐HDL) and from infected humans (AP‐HDL) on the oxidative metabolism of human polymorphonuclear leukocytes (PMN). Products of the H 2 O 2 –MPO–halide system were monitored by luminol‐enhanced chemiluminescence and superoxide anion formation was monitored by lucigenin‐enhanced chemiluminescence during stimulation of human PMN with phorbol myristate acetate (PMA) or an opsonized stimulus (OS). The results showed that N‐HDL and AP‐HDL affect the oxidative metabolism of PMN in different ways. The posible role of this effect is discussed. © 1997 John Wiley & Sons, Ltd.