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Determination of phenobarbital in plasma by micellar liquid chromatography
Author(s) -
MartínBiosca Y.,
Sagrado S.,
VillanuevaCamãnas R. M.,
MedinaHernández M. J.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(200004)14:2<113::aid-bmc935>3.0.co;2-5
Subject(s) - chemistry , chromatography , phenobarbital , detection limit , micellar liquid chromatography , plasma , analytical chemistry (journal) , micelle , aqueous solution , medicine , physics , quantum mechanics , pharmacology
A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl‐silane ODS‐2 C 18 analytical column, a guard column of similar characteristics, and a 0.03 M CTAB–3% 1‐propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 µg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7.5%. Copyright © 2000 John Wiley & Sons, Ltd.