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Measurement of albuterol in guinea pig serum by high performance liquid chromatography with fluorescence detection
Author(s) -
Loss James R.,
Orzechowski Raymond F.,
Hock Rick S.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(200002)14:1<1::aid-bmc926>3.0.co;2-i
Subject(s) - chemistry , chromatography , guinea pig , fluorescence , high performance liquid chromatography , medicine , physics , quantum mechanics
A sensitive, simple and reproducible high performance liquid chromatographic method for detecting and quantifying albuterol in guinea pig serum is described. A structurally related compound, bamethan, was used as an internal standard. The method employs ion‐pair extraction with di(2‐ethylhexyl)phosphate followed by chromatography on a Zorbax SB C18 reversed‐phase column. Fluorescence detection was used to identify the compounds of interest. The calibration curve was linear between 1 and 50 ng/mL albuterol hemisulfate salt (0.83 and 41.50 ng/mL albuterol base), and the limit of detection for a 1 mL sample was 1 ng/mL albuterol hemisulfate salt (0.83 ng/mL albuterol base). Serum levels of albuterol were quantified from guinea pigs that had received the drug by continuous subcutaneous infusion at a dose of 0.2 mg/kg/day for 1, 5 or 10 days, or 10 days followed by a 24 h washout period. Copyright © 2000 John Wiley & Sons, Ltd.