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Enantiomer‐specific high‐performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine
Author(s) -
AlDirbashi Osama,
Wada Mitsuhiro,
Kuroda Naotaka,
Inuduka Shigeko,
Nakashima Kenichiro
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199912)13:8<543::aid-bmc925>3.0.co;2-7
Subject(s) - chemistry , chromatography , metabolite , methamphetamine , enantiomer , urine , amphetamine , high performance liquid chromatography , reagent , fluorescence , pharmacology , biochemistry , stereochemistry , organic chemistry , dopamine , physics , medicine , quantum mechanics , neuroscience , biology
Enantiomer‐specific high‐performance liquid chromatography with fluorescence detection using 4‐(4,5‐diphenyl‐ 1H ‐imidazol‐2‐yl)‐benzoyl chloride as a fluorescence labeling reagent was applied to determine methamphetamine and its metabolites in abusers’ hair and urine. Hair samples were segmentally analyzed based on 1 cm long segments. In four hair samples, only the S (+)‐enantiomers of methamphetamine and its N ‐demethylated metabolite, S (+)‐amphetamine were detected. Satisfactory correlation ( r = 0.901) between the results of high‐performance liquid chromatography‐fluorescence and those of gas chromatography‐nitrogen phosphorous detection was obtained ( n = 19). In an abuser's urine sample, the S (+)‐ and R (−)‐enantiomers of methamphetamine, amphetamine and para ‐hydroxymethamphetamine were detected. The degree of N ‐demethylation of S (+)‐methamphetamine into the corresponding metabolite of amphetamine was significantly higher than that of the R (−)‐enantiomer. Copyright © 1999 John Wiley & Sons, Ltd.