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Determination of berberine in Rhizoma coptidis and its preparations by non‐aqueous capillary electrophoresis
Author(s) -
Ji Songgang,
Chai Yifeng,
Zhang Guoqing,
Wu Yutian,
Liang Dongsheng,
Xu Ziming
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199911)13:7<439::aid-bmc907>3.0.co;2-u
Subject(s) - berberine , chemistry , chromatography , capillary electrophoresis , acetic acid , aqueous solution , sodium acetate , reproducibility , methanol , sodium dodecyl sulfate , quantitative analysis (chemistry) , buffer solution , sodium , biochemistry , organic chemistry
A non‐aqueous capillary electrophoretic method was established for the determination of berberine in Rhizoma coptidis and its preparations. The effects of organic solvent and the concentrations of sodium acetate were studied, which showed that berberine in extracts of traditional Chinese medicine can be separated successfully in a buffer solution of 75 mmol/L of sodium acetate in methanol containing 1 mol/L of acetic acid. The simple and rapid method was linear in the range 25–200 µg/mL of berberine and had a good reproducibility, with the relative standard deviation below 2%. Non‐aqueous capillary electrophoresis is a satisfactory system for the analysis of alkaloids in traditional Chinese medicine. Copyright © 1999 John Wiley & Sons, Ltd.