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Determination of glycyrrhizin in Radix glycyrrhizae and its preparations by capillary zone electrophoresis
Author(s) -
Zhang Guoqing,
Ji Songgang,
Chai Yifeng,
Wu Yutian,
Yin Xueping
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199910)13:6<407::aid-bmc901>3.0.co;2-o
Subject(s) - glycyrrhizin , chromatography , chemistry , capillary electrophoresis , radix (gastropod) , reproducibility , glycyrrhiza , linear range , detection limit , pharmacology , alternative medicine , pathology , medicine , botany , biology
A capillary zone electrophoresis method was set up for the separation and determination of glycyrrhizin in Chinese medicinal preparations. Concentrations of Na 2 B 4 O 7 were optimized, which showed that glycyrrhizin in the sample could be separated from interference in the running buffer of 30 mmol/L Na 2 B 4 O 7 . Using declofenac as internal standard, the simple method was linear in the range 25–300 µg/mL of glycyrrhizin, and good reproducibility was obtained. The extracts of Radix glycyrrhizae and its preparations could be injected directly for analysis without any pretreatment. Copyright © 1999 John Wiley & Sons, Ltd.