Premium
Determination of fluoroquinolone residues in animal tissues by liquid chromatography
Author(s) -
Posyniak Andrzej,
Żmudzki Jan,
Semeniuk Stanislaw,
Niedzielska Jolanta,
Ellis Richard
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199906)13:4<279::aid-bmc844>3.0.co;2-a
Subject(s) - chromatography , chemistry , trichloroacetic acid , enrofloxacin , coefficient of variation , extraction (chemistry) , phosphoric acid , solid phase extraction , acetonitrile , reproducibility , protein precipitation , cartridge , ciprofloxacin , biochemistry , antibiotics , mechanical engineering , organic chemistry , engineering
A simple liquid chromatographic (LC) method was developed for the determination of fluoroquinolones (ciprofloxacin, difloxacin, enrofloxacin and sarafloxacin) in animal tissues. Isolation of fluoroquinolones from biological matrices was performed with 5% trichloroacetic acid‐acetonitrile (7:3) solution. For clean‐up, solid‐phase extraction with an SDBI (styrene–divinylobenzene) cartridge was used. LC analyses were performed with analytical column (LiChrospher 100 RP‐8 5 µm) and mobile phase (0.025 M O ‐phosphoric acid–acetonitrile 70:30, v/v) in ion‐pair mode. The whole procedure was validated in intra‐ and inter‐assay reproducibility and accuracy determination by simultaneously assaying of muscle, liver and kidney samples supplemented with fluoroquinolones at the level of 30 and 60 ng/g, respectively. The statistical evaluation demonstrates high absolute recovery (>80%) and low coefficient of variation (<10%) for all analysed samples. The detection limits for fluoroquinolones were 5 ng/g in muscle, liver and kidney samples. Copyright © 1999 John Wiley & Sons, Ltd.