Premium
A simple chiral high‐performance liquid chromatographic method to study the enantiomer‐differentiating action of microorganisms. An assay with DL ‐tryptophan
Author(s) -
Šoltés Ladislav,
Sébille Bernard
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199905)13:3<249::aid-bmc831>3.0.co;2-r
Subject(s) - chromatography , chemistry , high performance liquid chromatography , enantiomer , tryptophan , elution , column chromatography , silica gel , phosphate buffered saline , human serum albumin , bovine serum albumin , phosphate , amino acid , biochemistry , organic chemistry
To investigate the ‘enantiomer‐differentiating’ action of the microorganisms colonizing a phosphate‐buffered DL ‐tryptophan solution, a novel chiral high‐performance liquid chromatographic (HPLC) arrangement was developed and established. As the HPLC stationary phase, bovine serum albumin (BSA) bonded silica gel was used. In the function of the mobile phase, phosphate‐buffered DL ‐tryptophan solution was applied. The composition of the eluate was monitored by an HPLC spectrophotometric detector. After injecting the assayed sample into the eluent stream, the content of each tryptophan enantiomer was evaluated either from the positive or negative responses of the HPLC detector. The validity and the performance of this novel approach were confirmed by applying another chiral HPLC device working with human serum albumin (HSA) bonded silica gel as the stationary phase and with 1‐propanol containing phosphate buffer as the eluent. Copyright © 1999 John Wiley & Sons, Ltd.