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Determination of the relative impact of chromatographic conditions and solute structures on the retention behaviour of non‐ionic surfactants in RP‐HPLC
Author(s) -
Balcan Marieta,
Cserháti Tibor,
Forgács Esther,
Anghel DanFlorin
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199905)13:3<225::aid-bmc824>3.0.co;2-x
Subject(s) - chemistry , moiety , ethylene oxide , acetonitrile , chromatography , ionic strength , methanol , polar , high performance liquid chromatography , ionic bonding , oxide , organic chemistry , copolymer , ion , aqueous solution , physics , astronomy , polymer
The retention behaviour of non‐ionic surfactants containing different hydrophobic moiety and various lengths of polar ethylene oxide chain was studied on RP‐8 and RP‐18 columns using methanol and acetonitrile as organic modifiers. The relative impact of the chromatographic conditions on the retention strength was determined using stepwise regression analysis. Non‐ionic surfactants can be separated according to the character of the hydrophobic moiety, but not according to the length of the polar ethylene oxide chain. Calculations proved that the organic modifier exerted the highest impact on the retention; the effect of the number of carbon atoms in the hydrophobic moiety, column temperature and the length of the hydrophobic ligand on the surface of silica support are of secondary importance. Copyright © 1999 John Wiley & Sons, Ltd.