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Determination of ferrocene‐a in the blood serum of rabbits using reversed‐phase microcolumn hplc
Author(s) -
Pochkhidze Manana Sh.,
Asatiani Levan P.,
Rukhadze Marina D.,
Chitiashvili Zaza J.,
Tsartsidze Murad A.
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<89::aid-bmc812>3.0.co;2-8
Subject(s) - chemistry , chromatography , high performance liquid chromatography
Ferrocene‐A (ferrocenyl‐1‐phenyl‐1‐dioxy‐1,4‐butin‐2) has shown significant antitumour and antibacterial activity. To facilitate studies in this field a simple and rapid liquid chromatographic procedure was developed, allowing the determination of ferrocene‐A in the blood serum. The procedure was based on a reversed‐phase high‐performance liquid chromatography (HPLC) method, with UV detection at 210 nm. The mobile phase ethanol–0.01 M KH 2 PO 4 60:40 (v/v) provides the complete separation of ferrocene‐A, internal standard and endogenous compounds of serum. The recovery of ferrocene‐A from serum using a hexane‐isoamyl alcohol mixture was 90%. The proposed method is convenient for pharmacological and pharmacokinetic applications. Copyright © 1999 John Wiley & Sons, Ltd.