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Development of an hplc method for determining the alpha 2 ‐adrenergic receptor agonist brimonidine in blood serum and aqueous humor of the eye
Author(s) -
Karamanos N. K.,
Lamari F.,
Katsimpris J.,
Gartaganis S.
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<86::aid-bmc816>3.0.co;2-5
Subject(s) - brimonidine , chemistry , chromatography , aqueous humor , high performance liquid chromatography , aqueous solution , agonist , adrenergic agonist , pharmacology , glaucoma , ophthalmology , receptor , medicine , organic chemistry , biochemistry
A procedure for determining brimonidine [5‐bromo‐6‐(2‐imidazolidinylideneamino) quinoxaline] in biological samples using a reversed‐phase isocratic HPLC method is described. The application in blood serum and eye aqueous humor of patients treated with the Alphagan TM ophthalmic solution was carried out by enrichment of samples in brimonidine with solid‐phase liquid extraction. Brimonidine reached maximum levels in aqueous humor and serum within 2–2.5 h, whereafter a declining pattern was obtained. An approximate 50% level of brimonidine was identified in serum at 12 h after ocular administration, whereas in aqueous humor this percentage was determined after a period of 4–5 h. Copyright © 1999 John Wiley & Sons, Ltd.