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Studies on the determination of diacetyl guanfubase a concentration in rabbit plasma and pharmacokinetics
Author(s) -
Wang Weihong,
Wang Rubin,
Zhang Junren,
Liu Chuanhua,
Pan Shuju
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<83::aid-bmc805>3.0.co;2-i
Subject(s) - chemistry , pharmacokinetics , chromatography , diacetyl , rabbit (cipher) , plasma , pharmacology , biochemistry , medicine , statistics , physics , mathematics , quantum mechanics
A gas chromatographic method for monitoring diacetyl guanfubase A in plasma is described. The procedure involved a single solvent extraction of drug from rabbit plasma into ethyl acetate with guanfubase A as an internal standard. The extract was analyzed subsequently on a gas chromatograph equipped with a hydrogen flame ionization detector. The recovery was 86.43% ± 6.90% (±SD); the RSD of within‐day and between‐day was 2.81%–5.26% and 5.22%–8.24%, respectively; the regression line was linear over the concentration range of 25–200 µg/mL, the limit of detection was 10 µg/mL. No endogeneous interference was found in chromatograms of the biological samples. This method was applied to the pharmacokinetic study of diacetyl guanfubase A in rabbits. Copyright © 1999 John Wiley & Sons, Ltd.