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Ion exchange chromatography of aluminum using 3‐carboxy‐2‐naphthylamine‐ n,n ‐diacetic acid as a fluorescent post‐column chelating reagent
Author(s) -
Miyahara Taketsune,
Kitamura Hisayo,
Narita Kusuo,
Toyo’oka Toshimasa
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<70::aid-bmc818>3.0.co;2-i
Subject(s) - chemistry , reagent , chelation , chromatography , fluorescence , ion chromatography , ion , ion exchange , aluminium , column chromatography , inorganic chemistry , organic chemistry , physics , quantum mechanics
Ion exchange chromatography of aluminum ion using 3‐carboxy‐2‐naphthylamine‐ N,N ‐diacetic acid (CNDA) as a fluorescent post‐column chelating reagent was studied. The solution containing ammonium chloride and hydrochloric acid was used for the eluent, and acetate buffer solution containing CNDA was used for the post column chelating reagent. The peak of aluminum was separated from that of calcium, magnesium and zinc, and the chromatogram was not affected by copper(II) and iron(III). The calibration curve gave linear plots with a range of 0.0027–0.54 ppm aluminum, the regression coefficient of correlation ( r 2 ) was 1.000, and the detection limit ( S / N = 3) was 0.3 ppb, indicating that the method could determine aluminum with high sensitivity. It was demonstrated that CNDA is a useful metallofluorescent reagent for aluminum. This method has been successfully applied to the determination of aluminum in some tea drinks. Copyright © 1999 John Wiley & Sons, Ltd.