Premium
Buffer effect on the kinetics of ornithine carbamyl transferase by hplc
Author(s) -
Carunchio V.,
Girelli A. M.,
Messina A.
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<65::aid-bmc813>3.0.co;2-j
Subject(s) - chemistry , tris , chromatography , hydroxymethyl , triethanolamine , ornithine , citrulline , kinetics , transferase , high performance liquid chromatography , buffer (optical fiber) , stereochemistry , arginine , enzyme , biochemistry , analytical chemistry (journal) , amino acid , physics , quantum mechanics , telecommunications , computer science
A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus faecalis has been carried out, employing a new HPLC method based on the direct determination of citrulline. A different kinetic pattern was observed in tris(hydroxymethyl)aminomethane (TRIS) and triethanolamine (TEA) buffers: a ping‐pong or an ordered sequential mechanism are suggested, respectively. The inhibition of Tris buffer on OCT reaction has also been demonstrated. The inhibition of Tris buffer on OCT reaction has also been demonstrated. Copyright © 1999 John Wiley & Sons, Ltd.