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Direct injection of plasma to determine pseudoephedrine by high performance liquid chromatography with column switching
Author(s) -
Guo Ping,
Li Zhangwan,
Li Twuen,
Wang Xiaomin,
Li Famei
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<61::aid-bmc819>3.0.co;2-d
Subject(s) - chemistry , chromatography , pseudoephedrine , distilled water , elution , extraction (chemistry) , high performance liquid chromatography , ammonium acetate , detection limit , plasma , acetonitrile , ephedrine , physics , quantum mechanics , neuroscience , biology
An HPLC method has been developed for the determination of pseudoephedrine in plasma using column switching. Preparation of the sample was simple in that only 1000 µL of water was added to 200 µL of plasma before injection. A 900 µL aliquot was injected onto the precolumn. Double distilled water was used to elute and remove proteins and polar components in the sample. The components retained on the precolumn were flushed forward onto the analytical column by the mobile phase (acetonitrile–0.2 mol/L ammonium sulphate, 10:90 v/v) with automated column switching. The limit of determination of pseudoephedrine in plasma was 12 ng/mL. The relative standard deviations of intra‐ and inter‐assay for the determination of pseudoephedrine in plasma were 1.2–9.8% over the concentration range 1020–21.8 ng/mL. The mean recovery by on‐line solid phase extraction was 94.76% (RSD = 1.1%). Copyright © 1999 John Wiley & Sons, Ltd.