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Analysis of n ‐acetyl and n ‐glycolylneuraminic acid in rat serum and tissues with walker 256 carcinoma by high‐performance liquid chromatography
Author(s) -
Makatsori E.,
Aletras A.,
Karamanos N. K.,
Tsegenidis T.
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<57::aid-bmc815>3.0.co;2-8
Subject(s) - chemistry , chromatography , high performance liquid chromatography , carcinoma , medicine
Serum and tissue specimens from healthy Wistar rats and from rats with Walker 256 carcinoma were analysed for N ‐acetyl and N ‐glycolylneuraminic acid by high performance liquid chromatography (HPLC) as per‐ O ‐benzoylated derivatives. Both neuraminic acids were identified, while N ‐acetylneuraminic acid was the predominant sialic acid. Samples from rats with generalized metastasis showed a significant increase (45–80%) of total sialic acids. This phenomenon in serum is caused by the overproduction of sialic acids, as a result of synthesis of both types of neuraminic acids to a similar molar ratio. The increase of sialic acids in rat bones with metastatic cancer is mainly because of increased N ‐acetylneuraminic acid synthesis. These results suggest that the molecular mechanisms responsible for cancer metastasis in different tissues may be closely associated with increased synthesis of dominating neuraminic acid. Copyright © 1999 John Wiley & Sons, Ltd.