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Direct injection hplc analysis of some non‐steroidal anti‐inflammatory drugs on restricted access media columns
Author(s) -
Haque Ahsanul,
Stewart James T.
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<51::aid-bmc814>3.0.co;2-t
Subject(s) - ketoprofen , chemistry , chromatography , analyte , high performance liquid chromatography , probenecid , detection limit , calibration curve , pharmacology , medicine
Direct serum injection methods are described for the HPLC determination of selected non‐steroidal anti‐inflammatory drugs (NSAIDS) on restricted access media (RAM) columns, using either a Pinkerton (ISRP) or semi‐permeable surface (SPS) column. Twenty microlitres of a filtered serum sample was injected directly onto each column. Calibration curves were prepared for ketoprofen (1–20 µg/mL), fenbufen (0.8–20 µg/mL), sulindac (0.8–20 µg/m L ) and probenecid (1.5–40 µg/mL) on the SPS column, and nabumetone (1.5–40 µg/mL) and indomethacin (1.5–40 µg/mL) on the Pinkerton column. The percentage error and imprecision of the methods were found to be less than 10%. The inter‐ and intra‐day variability for analytes were in the range of 0.15–10%. The limits of quantitation and detection were in the range of 800–1500 and 300–800 ng/mL, respectively, for the analytes studied. A hydrophobic shielded phase (Hisep) column was also investigated and was found to be generally too retentive (>29 min) for assay of these analytes. Copyright © 1999 John Wiley & Sons, Ltd.