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Chiral gas chromatographic assay with flame ionization detection for amphetamine enantiomers in microsomal incubates
Author(s) -
Zeng S.,
Zhang L.,
Chen Y. Z.
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<33::aid-bmc809>3.0.co;2-b
Subject(s) - chemistry , chromatography , enantiomer , derivatization , microsome , amphetamine , detection limit , diastereomer , flame ionization detector , gas chromatography , high performance liquid chromatography , enzyme , organic chemistry , neuroscience , dopamine , biology
A chiral assay for amphetamine enantiomers in rat liver microsomal incubates is based on derivatization with ( S )‐(−)‐ N ‐(trifluoroacetyl)‐prolyl chloride (S‐TFPC), capillary chromatographic separation of the diastereomeric amide derivatives, and detection by a flame ionization detector. The method is capable of detecting low levels of S ‐ or R ‐amphetamine. The assay is linear from 5 to 250 µg/mL for each enantiomer, and the limit of detection is 0.5 µg/mL. The analytical method affords the average recoveries of 77.53 ± 5.22% for R ‐amphetamine and 74.47 ± 3.08% for S ‐amphetamine. The method allows the study of the metabolic depletion of S ‐ and R ‐amphetamine in rat liver microsomal incubates. The time‐dependent concentration of amphetamine enantiomers in rat liver microsomes was determined, and the stereoselectivity of amphetamine phase I metabolism was observed. Copyright © 1999 John Wiley & Sons, Ltd.