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Capillary gc–ms determination of melatonin in several pharmaceutical tablet formulations
Author(s) -
AboulEnein Hassan Y.,
Doneanu Catalin,
Covaci Adrian
Publication year - 1999
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199902)13:1<24::aid-bmc765>3.0.co;2-i
Subject(s) - chemistry , chromatography , melatonin , derivatization , gas chromatography–mass spectrometry , trimethylsilyl , ethyl acetate , extraction (chemistry) , reproducibility , mass spectrometry , capillary gas chromatography , gas chromatography , capillary action , relative standard deviation , detection limit , organic chemistry , materials science , composite material , medicine
A simple capillary GC–MS method for the determination of melatonin from tablets is proposed. Melatonin ( N ‐acetyl‐5‐methoxytryptamine) was determined by gas chromatography–mass spectrometry after extraction from ground tablets with ethyl acetate and derivatization with N ‐methyl‐ N ‐trimethylsilyl‐heptafluorobutyramide (MTSHFBA). Splitless injection offers good reproducibility with a standard deviation of ±2%. The proposed method was applied to analyse the melatonin content from three different tablet formulations. Copyright © 1999 John Wiley & Sons, Ltd.