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Liquid chromatographic separation of some PTH‐amino acids
Author(s) -
Bhushan Ravi,
Agarwal Rachna
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199811/12)12:6<322::aid-bmc754>3.0.co;2-0
Subject(s) - chemistry , chromatography , acetonitrile , chloroform , tetrahydrofuran , high performance liquid chromatography , resolution (logic) , silica gel , solvent , chromatographic separation , phase (matter) , amino acid , organic chemistry , biochemistry , artificial intelligence , computer science
Liquid chromatographic studies on the separation of ten PTH‐amino acids were carried out using normal phase untreated silica gel plates, C‐18 RP precoated plates and RP‐HPLC. Resolution of a complex mixture of PTH‐amino acids was achieved using all the three types. Certain new successful solvent systems have been worked out in each case. HPLC was carried out with Lichrosphere 100 RP‐18 (5 μm) column. Acetonitrile and sodium acetate buffer of pH 4.0 was used for reversed phase chromatography while for normal phase TLC combinations of chloroform–acetonitrile and chloroform–tetrahydrofuran were applied. Copyright © 1998 John Wiley & Sons, Ltd.