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Separation of several free polyamines and their acetylated derivatives by ion‐pair reversed‐phase high performance liquid chromatography
Author(s) -
AboulEnein Hassan Y.,
AlDuraibi Ibrahim A.
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199809/10)12:5<291::aid-bmc749>3.0.co;2-4
Subject(s) - chemistry , putrescine , spermidine , spermine , chromatography , dansyl chloride , detection limit , derivatization , polyamine , resolution (logic) , acetylation , chloride , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , enzyme , biochemistry , artificial intelligence , computer science , gene
Free polyamines and their acetylated derivatives, [putrescine (Put), spermine (Spm), spermidine (Spd), N 1 ‐acetylspermidine N 1 ‐AcSpd), N 8 ‐acetylspermidine N 8 ‐AcSpd)], were separated using ion‐pair reversed‐phase liquid chromatography after pre‐column derivatization with dansyl chloride. Also 1,6‐hexanediamine (1,6‐Hda) and 1,7‐diaminoheptane (1,7‐Dah) which used as an internal standards were separated under the same chromatographic conditions. The reported method provided a good resolution between N 1 ‐AcSpd and N 8 AcSpd ( Rs = 0.86). This quality of resolution together with the very low detection limit obtained, (33.6, 8.33, 2.72, 2.67, 4.76, 4.27, and 2.27 fmoles/μl for N 1 ‐AcSpd, N 8 ‐AcSpd, Put, 1,6‐Hda, 1,7‐Dah, Spm, and Spd, respectively), offer advantages to this method. Furthermore, a significant correlation coefficients were obtained as follows: 0.998 ( N 8 ‐AcSpd), 0.996 N 1 ‐AcSpd), 0.997 (Put), 0.9995 (1,6‐Hda), 0.9992 (1,7‐Dah), 0.9996 (Spd), and 0.997 (Spm). The described method can be adapted for the separation of the free and acetylated polyamines in biological specimens. © 1998 John Wiley & Sons, Ltd.