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Determination of quercetin in human plasma by HPLC with spectrophotometric or electrochemical detection
Author(s) -
Jones D. J. L.,
Lim C. K.,
Ferry D. R.,
Gescher A.
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199807/08)12:4<232::aid-bmc740>3.0.co;2-1
Subject(s) - chemistry , chromatography , detection limit , quercetin , ammonium acetate , methanol , high performance liquid chromatography , human plasma , absorption (acoustics) , ammonium , plasma , biochemistry , organic chemistry , physics , acoustics , antioxidant , quantum mechanics
A reversed‐phase high‐performance liquid chromatographic method for the determination of quercetin in human plasma following intravenous infusion is described. Quercetin in plasma was extracted with methanol–dimethyl sulphoxide (4:1 v/v) and separated on a C 18 Hypersil‐BDS column with 44% (v/v) methanol in 0.1 m ammonium acetate (pH 5.15) containing 0.27 m m EDTA as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 375 nm, or electrochemically, with a detection limit of 80 ng/mL and 2 ng/mL, respectively. © 1998 John Wiley & Sons, Ltd.