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Chiral resolution of four major flavanones in post‐administrative urine of chinese herbal medicines by HPLC on macroporous silica gel coated with cellulose tris(3,5‐dimethylphenylcarbamate)
Author(s) -
Li Chaun,
Homma Masato,
Oka Kitaro
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199807/08)12:4<199::aid-bmc735>3.0.co;2-0
Subject(s) - chemistry , chromatography , enantiomer , flavanone , silica gel , high performance liquid chromatography , resolution (logic) , cellulose , tris , urine , organic chemistry , flavonoid , biochemistry , artificial intelligence , computer science , antioxidant
Direct chiral resolution of four major flavanones recovered from post‐administrative urine of the traditional Chinese medicines Daisaiko‐to and Shosaiko‐to was achieved by high‐performance liquid chromatography (HPLC) on macroporous silica gel coated with cellulose tris(3,5‐dimethylphenylcarbamate), Chiralcel OD. Chromatographic conditions were optimized so as to attain satisfactory enantiomeric resolution of the polysubstituted flavanones. Urinary liquiritigenin and naringenin were considerable mixtures of R and S ‐enantiomers, while S ‐enantiomers of dihydrowogonin and dihydrooroxylin A were predominantly excreted. Our chiral HPLC techniques can be applied to pharmacokinetical evaluation of the chiral flavanone enantiomers following oral administration of the herbal medicines. © 1998 John Wiley & Sons, Ltd.