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Advantages of capillary electrophoresis for determination of choline in pharmaceutical preparations
Author(s) -
Lambert A.,
Colin J. L.,
Leroy P.,
Nicolas A.
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199805/06)12:3<181::aid-bmc803>3.0.co;2-5
Subject(s) - chemistry , capillary electrophoresis , chromatography , analyte , electrolyte , choline , betaine , selectivity , electrophoresis , capillary action , analytical chemistry (journal) , biochemistry , materials science , electrode , composite material , catalysis
Assay of choline in pharmaceutical preparations was realized by capillary zone electrophoresis (CZE) coupled with indirect UV detection. The suitability of several background electrolytes was investigated to optimize the separation of choline from other components such as amino acids, betaine and cations. Final operating conditions were as follows: a 75 μm × 50 cm uncoated fused‐silica capillary with an electrolyte consisting of 5 m M creatinine pH 3.2, a voltage of 25 kV, a temperature of 25°C and an UV detection at 210 nm. Choline migrates in less than 5 min and full selectivity vs other analytes was achieved. Validation data compared with those obtained with HPLC demonstrated the interest of CZE. © 1998 John Wiley & Sons, Ltd.