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Determination of cyclohexamone after derivatization with 2,4‐dinitrophenyl hydrazine in intravenous solutions stored in PVC bags by high performance liquid chromatography
Author(s) -
Khalfi F.,
Dine T.,
Luyckx M.,
Gressier B.,
Brunet C.,
Ballester L.,
Cazin M.,
Cazin J. C.
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199803/04)12:2<69::aid-bmc723>3.0.co;2-0
Subject(s) - chemistry , dinitrophenyl , derivatization , chromatography , hydrazine (antidepressant) , high performance liquid chromatography , antibody , immunology , biology
A high performance liquid chromatographic procedure is described for the determination of cyclohexanone leached in intravenous solutions from the poly(vinyl chloride) bags. After derivatization with 2,4‐dinitro‐ phenylhydrazine and extraction with pentane, the cyclohexanone derivative was analysed on a C18 BDS Hypersil column using mobile phase mixture of acetonitrile:water (55:45). Ultra‐violet detection was performed at 368 nm. The limit of quantification was 30 ng/mL and the assay was linear from 0.05 to 50 μg/mL. The recovery was better than 95%. The proposed method is satisfactory in its accuracy and precision with particularly relative standard deviations (RSD) for intra‐assay and inter‐assay of below 10%. This method has been successfully used for the determination of cyclohexanone in aqueous solutions such as sodium chloride (0.9%) and glucose (5%) stored in PVC containers. The values obtained varied between 2.04 and 44.9 μg/mL according to solutions and volume. © 1998 John Wiley & Sons, Ltd.