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3‐bromomethyl‐propyphenazone as a new derivatization reagent for high performance liquid chromatography of captopril and hydrochlorothiazide with UV‐detection
Author(s) -
Khedr Alaa,
ElSherief Hosny
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199803/04)12:2<57::aid-bmc720>3.0.co;2-g
Subject(s) - chemistry , chromatography , derivatization , reagent , captopril , high performance liquid chromatography , ethyl acetate , anhydrous , hydrochlorothiazide , enantiomer , organic chemistry , medicine , blood pressure , radiology
3‐Bromomethyl‐propyphenazone (BMP) was used as a derivatization reagent for the detection and quantification of captopril (CAP) and hydrochlorothiazide (HCT) by high performance liquid chromatography using Zorbax C8 column, and 0.05 m sodium acetate, acetonitrile, methanol (14:17:4; pH 6.5) as mobile phase system with UV‐detection at 254 nm. The cited reagent reacts with the mercapto and amino groups of CAP and HCT in acetone using anhydrous potassium carbonate as hydrobromide acceptor. The reaction was completed within 30 min for CAP and 60 min for HCT with heating at 105±5°C in mini‐reaction vial. The linear concentration ranges for both CAP and HCT were 8 to 160 and 6 to 140 ng per injection, respectively. The derivatized captopril was synthesized and confirmed with spectral analysis. This method was applied for determination of spiked captopril in human urine after extraction with Extrelut‐20 column using ethyl acetate:isopropanol (85:15 v/v) as eluant. © 1998 John Wiley & Sons, Ltd.