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Determination of nimesulide and hydroxynimesulide in human plasma by high performance liquid chromatography
Author(s) -
Glachetti Claudio,
Tenconi Anna
Publication year - 1998
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199803/04)12:2<50::aid-bmc719>3.0.co;2-b
Subject(s) - nimesulide , chemistry , chromatography , metabolite , high performance liquid chromatography , human plasma , methanol , detection limit , biochemistry , organic chemistry
Two specific methods for the simultaneous determination of nimesulide, a non steroidal anti‐inflammatory drug, and its hydroxylated metabolite in human plasma are described. Adopting a high performance liquid chromatographic (HPLC) system with UV detection (230 nm), the compounds, extracted from plasma in acidic medium, were separated on ODS columns under gradient conditions, using a phosphate buffer solution and methanol as mobile phase. For each method column length, gradient rate and composition were appropriately selected. The limit of quantitation was 25 ng/mL for both compounds. The two methods were validated by intra‐day assays at three concentration levels and applied in kinetic studies in healthy volunteers, during which inter‐day assays were carried out confirming their feasibility. © 1998 John Wiley & Sons, Ltd.