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HPLC/circular dichroism detection using a split‐type flow cell for polarized photometric detector
Author(s) -
Yamamoto Atsushi,
Matsunaga Akinobu,
Hayakawa Kazuichi,
Nishimura Masayuki
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199711)11:6<362::aid-bmc693>3.0.co;2-0
Subject(s) - chemistry , circular dichroism , chromatography , detector , stopped flow , analytical chemistry (journal) , optics , crystallography , physics , kinetics , quantum mechanics , reaction rate constant
HPLC/circular dichroism detection using a conventional spectrophotometric detector has been established. A split‐type flow cell developed for a polarized photometric detector, which is a non‐modulated polarimeter, was inserted into this detector. When two rays of circularly polarized light with opposite rotational senses to each other were incident in both cells by combining a polarizer and a 1/4 wave retarder in front of the cell assembly, the output from this spectrophotometric detector became solely the signal based on circular dichroism. Selective detection of a chiral analyte, in which the ratio of the difference in the absorbance between two rays mentioned above and the absorbance by normal rays is over 1/1000, is feasible using the proposed instrument. © 1997 John Wiley & Sons, Ltd.